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cell lines 369 primary human pulmonary microvascular endothelial cells hpmec  (PromoCell)


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    PromoCell cell lines 369 primary human pulmonary microvascular endothelial cells hpmec
    Cell Lines 369 Primary Human Pulmonary Microvascular Endothelial Cells Hpmec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+microvascular+endothelial+cells+hpmec/Human+Pulmonary+Microvascular+Endothelial+Cells/pm42262358-129-10-20
    Average 96 stars, based on 162 article reviews
    cell lines 369 primary human pulmonary microvascular endothelial cells hpmec - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Carcinoembryonic Antigen-Related Cell Adhesion Molecules (CEACAM) 1, 5 and 6 as Biomarkers in Pancreatic Cancer
    Article Snippet: Laminar flow experiments were performed using IBIDI microslides VI (IBIDI, Munich, Germany) connected to a syringe pump (Model 100 Series; kdScientific, Holliston, MA) and cell movement was observed with an inverted microscope (Zeiss, Jena, Germany; Axiovert 200). .. Tumor cells were suspended in cell culture medium (20 ml, 200 000 cells/ml) and microslides were coated with Human Pulmonary Microvascular Endothelial Cells (HPMEC) (PromoCell, Heidelberg, Germany). ..

    Article Title: Multifaceted activation of STING axis upon Nipah and measles virus-induced syncytia formation
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMEC) [ ] from a male donor were cultured in Endothelial Cell Growth Medium GM MV2 (Promocell) and SupplementalPack (5% FCS, 5 ng/ml recombinant hEGF, 10 ng/ml recombinant human bFGF, 20 ng/ml long R3 IGF, 0.5 ng/ml recombinant hVEGF, 1 μg/ml ascorbic acid), in flasks coated with 0.1% bovine gelatine (Sigma) in PBS. .. For infection, HPMECs were plated at 2.5x10 5 cells/well in 12-well plates or at 5x10 4 cells/well in 8-well Ibidi slides and cultured with NiV-M-eGFP at a MOI of 1 or 3 or MeV-EdmH-eGFP at a MOI of 1 PFUs/cell for 48 h. 293T cells (ATCC) were cultured in DMEM supplemented with 10% FBS, 1% HEPES and 1% penicillin-streptomycin.

    Recombinant:

    Article Title: Multifaceted activation of STING axis upon Nipah and measles virus-induced syncytia formation
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMEC) [ ] from a male donor were cultured in Endothelial Cell Growth Medium GM MV2 (Promocell) and SupplementalPack (5% FCS, 5 ng/ml recombinant hEGF, 10 ng/ml recombinant human bFGF, 20 ng/ml long R3 IGF, 0.5 ng/ml recombinant hVEGF, 1 μg/ml ascorbic acid), in flasks coated with 0.1% bovine gelatine (Sigma) in PBS. .. For infection, HPMECs were plated at 2.5x10 5 cells/well in 12-well plates or at 5x10 4 cells/well in 8-well Ibidi slides and cultured with NiV-M-eGFP at a MOI of 1 or 3 or MeV-EdmH-eGFP at a MOI of 1 PFUs/cell for 48 h. 293T cells (ATCC) were cultured in DMEM supplemented with 10% FBS, 1% HEPES and 1% penicillin-streptomycin.

    Incubation:

    Article Title: Highly potent quinoxalinediones inhibit α-hemolysin and ameliorate Staphylococcus aureus lung infections.
    Article Snippet: Hemoglobin release from lysis of erythrocytes was determined by measuring the optical density at 540 nm using a Biotek microplate reader (Agilent). .. Human pulmonary microvascular endothelial cells (HPMEC) from Promocell were grown on 8-well coverslips and incubated with 1 mg/ml Hla for 30 min in the absence or presence of H831 or H052. ..



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    HPMECs were treated with 1 µg/mL of LPS for different time. A Cell viability in HPMECs. B The concentration of TNF-α. C The concentration of IL-6. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001

    Journal: BMC Pediatrics

    Article Title: MiR-483-5p has the potential to serve as a biomarker in perioperative respiratory adverse events in children under general anesthesia

    doi: 10.1186/s12887-025-06483-x

    Figure Lengend Snippet: HPMECs were treated with 1 µg/mL of LPS for different time. A Cell viability in HPMECs. B The concentration of TNF-α. C The concentration of IL-6. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001

    Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs) were provided by PromoCell (C-12281, Heidelberg, Germany) and cultured in endothelial cell medium (ECM) supplemented with FBS, antibiotics, and endothelial cell growth serum.

    Techniques: Concentration Assay